Chemical and immunochemical analyses of Bacteroides fragilis lipopolysaccharides
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چکیده
منابع مشابه
Chemical and immunochemical analyses of Bacteroides fragilis lipopolysaccharides.
Lipopolysaccharides (LPSs) from 17 different Bacteroides fragilis strains were extracted in a two-step procedure. The first step was a hot phenol-water extraction of whole bacteria, resulting in a crude aqueous phase, which after lyophilization in a second step was extracted with a phenol-chloroform-light petroleum mixture. The resulting LPSs, which were essentially free from contaminating nucl...
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Fermentor growth of Bacteroides fragilis under controlled conditions in a complex medium containing 1% glucose and 10% fetal calf serum resulted in high yields of bacteria. After hot phenol-water extraction of the organisms, capsular polysaccharide was isolated from the aqueous phase and purified by Sephacryl S-300 chromatography in a buffer with 3% sodium deoxycholate. Lipopolysaccharide was i...
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An immunochemical comparison of outer membrane antigens obtained from five select and biochemically defined strains indicated that there are several serotypes of Bacteroides fragilis. Each strain was serologically defined by individual or by combinations of determinant groups composed of carbohydrates in the form of polysaccharides or glycoproteins. The carbohydrate constituents were tentativel...
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Recognition of bacterial lipopolysaccharide (LPS) is critical in the host defence against Gram-negative infection. While enterobacterial LPS signals via Toll-like receptor 4 (TLR4), it has recently been reported that the LPS of Leptospira interrogans, Legionella pneumophila, Rhizobium species Sin-1 and at least one strain of Porphyromonas gingivalis are capable of signalling via TLR2. Using a T...
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Studies of three reference strains of Bacteroides fragilis subsp. fragilis showed that they grow well in a minimal defined medium containing glucose, hemin, vitamin B(12), minerals, bicarbonate-carbon dioxide buffer, NH(4)Cl, and sulfide. The vitamin B(12) requirement of 0.1 ng/ml was replaced with 7.5 mug of methionine. Cysteine or sulfide was an excellent source of sulfur, thioglycolate was a...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1985
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.49.1.197-201.1985